Their proud parents were said to be "overjoyed and delighted" by the births, which took place by Caesarian section, and went without a hitch.
One of the little girls weighed in at a bouncing 3.2 kilos while the other tipped the scales at 2.5 kilos.
Neither has yet been named but suggestions have included Joy, after the wife of the doctor who made it all possible, Joshua Noreh.
The new mothers first read about the local availability of the test tube process in the Daily Nation's Horizon magazine and that encouraged them to undergo the lengthy and complex treatment that has finally brought them so much happiness.
Both babies were born at around 11am at the private Avenue Hospital in Parklands, Nairobi, to mothers under Dr Noreh's care.
The births were supervised by a team of four specialist doctors and support staff and they revealed that a further seven test tube Kenyan babies are on the way.
's successful births came 28 years after the birth of the world's first test tube baby - Louise Brown, at Oldham, in the United Kingdom - and are the first to follow IVF treatment to Kenyan women carried out entirely in this country.
Other Kenyan women have had test tube babies after IVF treatment in Uganda and South Africa, for example.
The brand new parents were so anxious to have a family that they paid more than Sh300,000 each for the in vitro fertilisation procedure offered by Dr Noreh at his clinic in the city's Afya Centre.
The IVF programme involves taking an egg directly from an ovary of the mother-to-be, and fertilising it with sperm of the father in a special glass dish.
Dr Noreh looks through an ICSI machine that is used to inject a sperm into an egg.
Mother's womb
The fertilised human egg is then collected in a test tube where it is allowed to develop into an embryo, which takes around three days.
Next the embryo is implanted in the mother's womb, allowing for a normal gestation period of nine months followed by the birth.
The husband of one of the mothers, who had been waiting anxiously outside the operating theatre, told Dr Noreh: "I am ready to give you anything you ask for.
His wife, who said she wished at this stage to be known only as Jane - not her real name - and speaking moments before delivery, said they had waited for this moment for the last 10 years.
"This is the moment I have always waited for and for the first time in my life, I feel a great sense of relief and hope," she said, wiping a tear from her eyes.
And after the baby's safe delivery, Dr Noreh commented: "By these deliveries, it's opening future treatment of infertile couples and also indicates we can do it locally."
Dr Noreh's wife, Joy, who is a nurse at the clinic, added: "These women trusted that we can do something for them and we thank God for that. Even for those who did not conceive, they are part of this victory."
On the eve of the birth, Jane, who is aged 35, said she was very nervous, but the urge to deliver had been with her since she was three weeks' pregnant.
"I am just waiting for tomorrow to hold my baby," she said then. "That is all I want and what is on my mind now."
And she added: "This is going to be my longest night and I will force some sleep."
Jane went for treatment after failing to conceive and seeing a series of doctors who gave conflicting reasons for her problem.
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